Assay of anti-D using the Technicon AutoAnalyzer and the international standard anti-D typing serum

Assay of anti-D using the Technicon Auto-Analyzer and the international standard anti-D typing serum.

A method is described for the automated quantitation of the anti-D content of sera. This procedure is far more reproducible than current manual techniques. The use of the International Standard anti-D typing serum in the calibration of a working standard enables results to be expressed in mug/ml of antibody protein.

The adverse effect of bromelin in anti-D quantitation using the AutoAnalyzer.

With eight of 32 sera significantly higher estimations of anti-D concentration were obtained when test cells premodified with bromelin were used instead of bromelin as a reagent added to the reaction manifold. With one serum the difference in estimation, using the two techniques, revealed a ten-fold discrepancy. The consequences of these findings are discussed in relation to the use of the Auto...

Manipulative and inherent errors in anti-D quantitation using the AutoAnalyzer.

The limits of accuracy for the quantitation of anti-D using the AutoAnalyzer have been reduced from +/- 26% with manually prepared dilutions to +/- 14% using an automatic pipette, as expressed by the 95% confidence limits. The error inherent in the AutoAnalyzer was estimated to contribute +/- 10% to the overall error. Problems associated with the reproducibility of this method for anti-D quanti...

A capillary tube method of Rh typing using albumin aggluting anti-D serum.

An evaluation of the Technicon AutoAnalyzer

SYNOPSIS Obviously there is need for a reliable means of automating complement-fixation tests. This paper offers an account of experience with an AutoAnalyzer adapted for this purpose. Results of syphilis screening tests done by the AutoAnalyzer compared with those of tests performed manually showed satisfactory agreement, the automated method being slightly less specific in our hands. There we...